Our group associates expertise in nonlinear optics and tissue microscopy. We develop novel experimental approaches based on multimodal nonlinear microscopy (*), and their application to the study of developing tissues.
Modern issues in systems biology (development, neuroscience) require tissue-scale measurements of multiple cell parameters. Since its introduction in the early 90s, multiphoton microscopy has proven invaluable for tissue studies with its ability to provide micrometer-scale 3D resolution in intact, scattering samples. However tissue imaging is still challenging in terms of speed, depth, sensitivity, and number of accessible parameters.
We develop strategies to expand the capabilities of current microscopes (characterization of coherent contrast mechanisms; multimodal/multicolor imaging of several signals; light-sheet excitation for faster imaging; wavefront control for focus engineering and adaptive optics), and we explore novel applications such as imaging embryonic & nervous tissue development in animal models, and optical diagnostics.
(*) Combining photomanipulation and multiphoton contrasts such as multiphoton-excited fluorescence (2PEF, 3PEF), harmonic generation (THG, SHG), coherent anti-Stokes Raman scattering (CARS), four-wave mixing (FWM), fluorescence lifetime (FLIM), polarimetry.